Preparation of PLL-PEG-PLL and its application to DNA encapsulation
Identifieur interne : 000976 ( Istex/Checkpoint ); précédent : 000975; suivant : 000977Preparation of PLL-PEG-PLL and its application to DNA encapsulation
Auteurs : Zigang Yang [République populaire de Chine] ; Fen Zhou [République populaire de Chine] ; Jianjun Yuan [République populaire de Chine] ; Lixin Ma [République populaire de Chine] ; Chao Zhai [République populaire de Chine] ; Shiyuan Cheng [République populaire de Chine]Source :
- Science in China Series B: Chemistry [ 1006-9291 ] ; 2006-08-01.
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- topic : ADN.
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Abstract
Abstract: PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aqueous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10–35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.
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DOI: 10.1007/s11426-006-2006-9
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www.scichina.com</publisher><pubPlace>Beijing</pubPlace><date type="published" when="2006-08-01">2006-08-01</date><biblScope unit="volume">49</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="357">357</biblScope><biblScope unit="page" to="362">362</biblScope></imprint><idno type="ISSN">1006-9291</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1006-9291</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA</term><term>block copolymer</term><term>poly(L-Lysine)</term><term>poly(ethylene glycol)</term><term>polyion complex micelles (PIC)</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>ADN</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aqueous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10–35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Yang, Zigang" sort="Yang, Zigang" uniqKey="Yang Z" first="Zigang" last="Yang">Zigang Yang</name></region><name sortKey="Cheng, Shiyuan" sort="Cheng, Shiyuan" uniqKey="Cheng S" first="Shiyuan" last="Cheng">Shiyuan Cheng</name><name sortKey="Cheng, Shiyuan" sort="Cheng, Shiyuan" uniqKey="Cheng S" first="Shiyuan" last="Cheng">Shiyuan Cheng</name><name sortKey="Ma, Lixin" sort="Ma, Lixin" uniqKey="Ma L" first="Lixin" last="Ma">Lixin Ma</name><name sortKey="Yuan, Jianjun" sort="Yuan, Jianjun" uniqKey="Yuan J" first="Jianjun" last="Yuan">Jianjun Yuan</name><name sortKey="Zhai, Chao" sort="Zhai, Chao" uniqKey="Zhai C" first="Chao" last="Zhai">Chao Zhai</name><name sortKey="Zhou, Fen" sort="Zhou, Fen" uniqKey="Zhou F" first="Fen" last="Zhou">Fen Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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